Pdgfrb creer
Splet07. mar. 2024 · Mutation details : The mouse Pdgfb promoter was used to drive inducible expression of an improved cre gene fused to ERT2 and followed by an IRES-EGFP then an … Splet07. mar. 2024 · The experiments to validate the scRNAseq findings by whole mount immunofluorescent microscopy were performed in Pdgfrb-CreER (Jackson Laboratory #030201) , Acta2-Cre (Jackson Laboratory #029925) , and Acta2-CreER (a gift from Dr. Ivo Kaljzic at the University of Connecticut) all crossed into Ai9-tdTomato reporter models. …
Pdgfrb creer
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NG2+ vascular pericytes do not give rise to CAs. As PDGFRb lacks specificity to vascular mural cells, we generated another mouse line, NG2-CreER, by inserting CreER-P2A in frame with the translational start codon of the NG2 gene ( Figure 7 A) to examine the contribution of vascular pericytes to CAs. Splet01. jul. 2005 · Mature PDGFRB consist of 1067 amino acids and can bind strongly to PDGF-BB and -DD homodimers, but weakly to AB heterodimers and not to PDGF-AA homodimer. …
SpletThe Pdgfrb-Cre Mouse was developed in the laboratory of Ralf Adams at the London Research Institute. The model was generated by microinjecting a transgene containing a … Splet02. feb. 2024 · First, Pdgfrb-CreER;R26-tdTomato mice were treated with tamoxifen at P14, and hearts were harvested for targeting analyses at P21 (Figure 4 A). Immunostaining for …
Splet09. feb. 2024 · A suite of new Dre recombinase drivers markedly expands the ability to perform intersectional genetic targeting Highlights • More than 70 new Dre driver lines are provided as a resource for intersectional genetics • PDGFRa + PDGFRb + or PDGFRa + perivascular cells contribute to de novo adipocytes • SpletThe Pdgfrb-Cre line has been used as a tool to specifically target pericytes and vascular smooth muscle cells. Recent studies showed additional targeting of cardiac and …
SpletPdgfrb-CreER T2 , Hif1a (flox/flox) mice were induced with tamoxifen, rested, and then exposed to hypoxia for either 1 day to confirm HIF1-a deletion in PDGFR-b + cells ( Figure S2A) or 21 days to ...
Splet21. apr. 2024 · Upon carotid artery ligation, PDGFRa-positive (+) cells were slowly recruited to the neointima and exhibited an immature SMC phenotype. In contrast, in a more severe wire denudation injury, PDGFRa+... postinumero ylivieskaSplet10. nov. 2024 · Request PDF Characterization of the two inducible Cre recombinase-based mouse models NG2- CreER TM and PDGFRb-P2A-CreER T2 for pericyte labeling in the … postinumerohaku helsinkiSplet07. mar. 2024 · Mutation details : The mouse Pdgfb promoter was used to drive inducible expression of an improved cre gene fused to ERT2 and followed by an IRES-EGFP then an frt-flanked chloramphenicol resistance gene in vascular endothelium. The PAC used (RP21-629-J8) contains the entire Pdgfb gene with the cre cassette inserted immediately … postinumero ypäjäSpletWe have generated a novel mouse line expressing tamoxifen-inducible Cre-recombinase under the control of the platelet-derived growth factor receptor β promoter: PDGFRβ-P2A-CreER T2. We evaluated the expression of the PDGFRβ-P2A-CreER T2 line by crossing it with fluorescent reporter lines and analyzed reporter signal in the angiogenic retina ... postinumeroluettelo joensuuSplet032770 B6.129S- Pdgfra tm1.1(cre/ERT2)Blh /J Pdgfrα-CreER T2 mice have a cre/ERT2 fusion gene inserted into the start codon of the platelet derived growth factor receptor, … postinumerohaku jyväskyläSplet14. sep. 2016 · In the central nervous system (CNS) platelet derived growth factor receptor alpha (PDGFRα) is expressed exclusively by oligodendrocyte progenitor cells (OPCs), making the Pdgfrα promoter an ideal tool for directing transgene expression in this cell type. postinumeroluettelo helsinkiSplet11. nov. 2024 · Recently, however, an elegant study using Pdgfrb-CreER T2 to disrupt Srf in pericytes found defective migration of these cells during sprouting angiogenesis in the newborn retina, resulting in ... postinumeroluettelo oulu