Halolysin

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August undefined, 2024

WebJan 15, 2024 · Sequence (a) and crystal structure (b and c) of a polypeptide chain fragment from the surface layer protein tetrabrachion from Staphylothermus marinus showing the right-handed parallel RHCC tetramer. In (a) the sequence positions are labelled from a to k with the hydrophobic core positions highlighted in red. (b) Axial view from the N-terminus ... WebGenerally, HlyA from the extremely halophilic archaeon Hcc. salifodinae is an alkaliphilic and low salt-adapted halolysin with high activity, thus representing an attractive candidate for various industrial uses.

Halolysin SptA, a Serine Protease, Contributes to …

WebYet the diversity of halolysins remains underexplored. In this study, a halolysin from the extremely halophilic archaeon Haladaptatus sp. DYF46 (Hly Hap) was identified to be a … WebApr 15, 2024 · Except the halolysin from Hbt. halobium, the other characterized halolysins displayed the optimum activities at temperatures higher than 45 °C. The halolysin from Halococcus sp. GUGFAWS-3 exhibited high activities at a very broad temperature range of 40–80 °C. It displayed the optimum temperature of 70 °C, much higher than the optimum ... the wateredge inn

Staphylothermus marinus - an overview ScienceDirect Topics

WebJul 1, 2010 · Aims: The alkaliphilic haloarchaeon Natrialba magadii secretes a halolysin‐like protease (Nep) that is active and stable in high salt and in organic solvents, which represents a potential resource for biocatalysis in low water activity conditions. In this study, the effect of the growth stage on Nep biosynthesis was examined. Methods and … WebFeb 12, 2024 · This study shows that halolysin R4 from Haloferax mediterranei provides its host antagonistic and defensive activities against other haloarchaea, which expands the knowledge of the traditional function of halo archaeal extracellular proteases. This study shows that halolysin R4 from Haloferax mediterranei provides its host antagonistic and … WebAug 3, 2024 · Halolysin SptA is the major extracellular protease of Natrinema sp. J7-2, and its production is growth-phase dependent. The growth phase-dependent production of … the waterfall banquet hall in delaware

Halolysin SptA, a Serine Protease, Contributes to Growth-Phase ...

A novel halolysin without C-terminal extension from an …

WebMay 23, 1996 · A gene encoding a halophilic serine proteinase, halolysin R4, from a halophilic archaeon Haloferax mediterranei strain R4 was cloned, its nucleotide … Webjournals.asm.org the waterfall cafeWebJun 14, 2008 · The Hfx. volcanii synthesized protease was active in high salt, high pH and high DMSO. This study provides the first molecular characterization of a halolysin-like protease from alkaliphilic haloarchaea and is the first description of a recombinant system that facilitates high-level secretion of a haloarchaeal protease. the waterfall by rabindranath tagore summary

"WebJun 9, 2010 · Aims: The alkaliphilic haloarchaeon Natrialba magadii secretes a halolysin-like protease (Nep) that is active and stable in high salt and in organic solvents, which represents a potential resource for biocatalysis in low water activity conditions. In this study, the effect of the growth stage on Nep biosynthesis was examined. Methods and Results: … " - Halolysin

Halolysin

WebHalolysin SptA from haloarchaeon Natrinema sp. J7 consists of a subtilisin-like catalytic domain and a C-terminal extension (CTE) containing two cysteine residues. In this report, we have investigated the function of the CTE using recombinant enzymes expressed in Haloferax volcanii WFD11. Deletion of the CTE greatly reduced but did not abolish ... WebCiteSeerX - Document Details (Isaac Councill, Lee Giles, Pradeep Teregowda): The study used various screening techniques, clustering, decision tree and generalized rule induction (association) (GRI) models and molecular phylogenic relationship to search for patterns of halophilicy and to find features contribute to halolysin salt stability. We found that Met …

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WebApr 1, 2024 · In this study, a halolysin from the extremely halophilic archaeon Haladaptatus sp. DYF46 (HlyHap) was identified to be a novel type of halolysin without C-terminal … WebHalolysin SptA from haloarchaeon Natrinema sp. J7 consists of a subtilisin-like catalytic domain and a C-terminal extension (CTE) containing two cysteine residues. In this report, we have investigated the function of the CTE using recombinant enzymes expressed in Haloferax volcanii WFD11. Deletion of the CTE greatly reduced but did not abolish ...

WebApr 15, 2024 · Except the halolysin from Hbt. halobium, the other characterized halolysins displayed the optimum activities at temperatures higher than 45 °C. The halolysin from … WebAug 3, 2024 · Halolysin SptA is the major extracellular protease of Natrinema sp. J7-2, and its production is growth-phase dependent. The growth phase-dependent production of halolysin has been described in some haloarchaea such as Ncc. occultus (Studdert et al., 1997), Nab. asiatica (Kamekura and Seno, 1990), and Nab. magadii (Paggi et al., 2010).

WebFeb 12, 2024 · Halolysin has a long C-terminal extension of approximately 120 amino acids which has not been found in other extracellular subtilisin type serine proteases. The … WebA novel halolysin without C-terminal extension from an extremely halophilic archaeon Author: Jing Hou, Si-Ya Li, Yang-Jie Zhao, Heng-Lin Cui Source: Applied microbiology and biotechnology 2024 v.106 no.8 pp. 3009-3019 ISSN: 0175-7598 Subject:

WebHalolysin precursors contain a twin arginine signal pep-tide, a propeptide, a subtilase catalytic domain and a C-ter-minal extension (CTE). During or after the trans-membrane transport, they are autocatalytically cleaved to generate a mature halolysin. The mature halolysin is composed of a cat-alytic domain and a CTE (Du et al. 2015).

WebHalolysin precursors contain a twin arginine signal pep-tide, a propeptide, a subtilase catalytic domain and a C-ter-minal extension (CTE). During or after the trans-membrane … the wateredge hotelWebFeb 1, 2015 · In the present study, we showed that the Tat-dependent halolysin SptA matures autocatalytically and that premature activation of SptA in the heterologous host … the waterfall catering delawareWebMay 23, 1996 · @article{Kamekura1996HalolysinRA, title={Halolysin R4, a serine proteinase from the halophilic archaeon Haloferax mediterranei; gene cloning, expression and structural studies.}, author={Masahiro Kamekura and Yukio Seno and Michael Dyall-Smith}, journal={Biochimica et biophysica acta}, year={1996}, volume={1294 2}, pages={ … the waterfall diet pdfWebHemolysin is an important virulence factor in the extremely common E. coli infections of the urinary tract (UTI) and other extraintestinal sites [ 4,5,14] and is also produced by … the waterfall claymont deWebFeb 12, 2024 · Importance: This study shows that halolysin R4 from Haloferax mediterranei provides its host antagonistic and defensive activities against other haloarchaea, which expands our knowledge on the traditional function of haloarchaeal extracellular proteases. Haloarchaeal extracellular serine proteases have been previously discussed as growth … the waterfall dietWebThe gene of a halophilic alkaline serine protease, halolysin, from an unidentified halophilic archaea (archaebacterium) was cloned and its nucleotide sequence was determined. The deduced amino acid sequence showed that halolysin consists of 411 amino acids, with a molecular weight of 41,963. The highest homology was found with thermitase from … the waterfall cafe pittsburghWebNational Center for Biotechnology Information the waterfall diet reviews