WebMar 3, 2015 · Function and Optimization of the Detection System in LAB. We designed and tested a set of expression plasmids in L. lactis, originally characterized in E. faecalis, 18,25 for their ability to confer expression of a transcriptional lacZ reporter gene in response to exogenously supplied cCF10 peptide (Supporting Information Figure S1).Plasmid pBK2 … WebSep 6, 2005 · Here, we present the molecular structure of PrgX, a DNA- and peptide-binding protein that regulates expression of the conjugative transfer genes of the Enterococcus faecalis plasmid pCF10 in response to an intercellular peptide pheromone signal. Comparison of the structures of PrgX and the PrgX/pheromone complex suggests that …
In Vivo Induction of Virulence and Antibiotic Resistance Transfer in ...
WebApr 11, 2024 · 腾讯定性此前微信QQ故障为一级事故,多位高管遭处罚. 4月10日消息,此前3月29日凌晨,腾讯旗下的微信和QQ等业务曾出现崩溃状况,包括微信语音 ... WebCell-cell communication mediated by peptide pheromones (cCF10 [CF]) is essential for high-frequency plasmid transfer in vitro in Enterococcus faecalis. To examine the role of pheromone signaling in vivo, we established either a CF-pro-ducing (CF>) recipient or a recipient producing a biologically inactive variant of CF (CF> recipient) in a ... different eos memberships
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WebAsc10, the AS of the sex pheromone plasmid pCF10 (encoding tetracycline resistance) , is induced by the 7-amino-acid peptide cCF10 encoded by the ccfA gene. The donor cell … Web21 rows · Physical Properties of steel grade ASTM CF10. Temperature. (°C) Modulus of elasticity. (GPa) Mean coefficient of thermal expansion 10-6/ (°C) between 20 (°C) and. … WebIt is believed that cCF10 is an essential factor for conjugative plasmid transfer [55]. In the present study, we found that cCF10 (LVTLVFV, 20 ng/mL) and estradiol (0.01 nM β-E2 … different equations for slope